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Humanized Antibody Preparation Strategy

Refined antibody mainly relates to what is re-expressed from mouse monoclonal antibody by gene cloning and recombinant DNA technology, and most of the amino acid sequence is replaced by human sequence and the affinity and specificity of the monoclonal mouse parent is maintained and the antibody is degraded. Its heterogeneity benefits the human body. You can get information about antibodies from various sites like

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Chimeric antibodies

The variable region of the murine antibody gene was recombined with the constant region of the human antibody gene by recombinant DNA technology, and the recombinant gene was inserted into myeloma cells for expression.

Depending on the plasmid vector used to label the gene product, the antibiotic or suitable preparation used for screening, and the chimeric-secreting cell lines in human mice were cloned in a manner similar to conventional techniques.

Humane antibody transplanted to CDR

FR in region V of murine antibody still has some immunogenicity. These are very far from truly humanized antibodies, and some can produce a strong anti-idiotypic response.

To reduce the composition of mice, humans attempted to replace mouse FR with human FR to produce more comprehensive antibodies from the antibody humanization service, namely the three CDRs were the source of mice, all of which were human. also known as CDR graft antibody. Or modified antibodies.